Whole-genome resequencing of Bacillus cereus and expression of genes functioning in sodium chloride stress. (March 2017)

• Record Type: Journal Article
• Authors: Xu, Zhenbo; Xie, Jinhong; Liu, Junyan; Ji, Lili; Soteyome, Thanapop; Peters, Brian M.; Chen, Dingqiang; Li, Bing; Li, Lin; Shirtliff, Mark E.
• Is Part Of: Microbial pathogenesis. Volume 104(2017)
• Page Start: 248
• Page End: 253
• Publication Date: 2017-03
• Subjects: Bacillus cereus -- Resequencing -- K+ transport -- Glutathione peroxidase -- Salt-tolerance; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2017.01.040
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Bacillus cereus is one of the most common opportunistic pathogens responsible for various foodborn diseases. To investigate the regulatory mechanism of B. cereus under high osmotic pressure, two B. cereus strains B25 and B26 were isolated from the industrial soy sauce residue containing high-salt concentration. Resequencing was performed by Illumina/Solexa platform and 13, 646 SNPs and 434 InDels were identified as common variants between B25 and B26 against reference genome, followed by COG, GO, and KEGG enrichment analysis. Furthermore, 49 key genes involving in Na + /H +, K + transporter, dipeptide or tripeptide transporter, stress response were selected and classified into 27 groups. Further validation was performed by qRT-PCR, and 4 candidate genes were found most associated with osmotic response. Gene expression of the 4 candidate genes was then analyzed accordingly, and down regulation was obtained for gene BC0669 and BC0754 associated with K + transport system. However, dramatic up regulation was detected for gene BC2114 involving in glutathione peroxidase, indicating the activation of antioxidant responses by osmotic stress via genetic regulation. As concluded, bioinformatic analysis and gene expression profile represented the basis of further investigation on the genetic and regulatory mechanism of bacterial salt tolerance. Highlights: The maximum salt-tolerance capability of B25 and B26 was 20% and 15%, respectively. 13, 646 SNPs and 434 InDels were identified after resequencing. Two genes associated with K+ transport system were down-regulating. One genes associated with glutathione peroxidase was up-regulating.