Multi-antibiotic resistant and putative virulence gene signatures in Enterococcus species isolated from pig farms environment. (March 2017)

• Record Type: Journal Article
• Authors: Beshiru, Abeni; Igbinosa, Isoken H.; Omeje, Faith I.; Ogofure, Abraham G.; Eyong, Martin M.; Igbinosa, Etinosa O.
• Is Part Of: Microbial pathogenesis. Volume 104(2017)
• Page Start: 90
• Page End: 96
• Publication Date: 2017-03
• Subjects: Multidrug resistance -- Virulence gene -- Enterococci -- Pig farms; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2017.01.020
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: The continuous misuse of antimicrobials in food animals both orally and subcutaneously as therapeutic and prophylactic agents to bacterial infections could be detrimental and contribute to the dissemination of resistant clones in livestock production. The present study was carried out to determine the antibiogram and virulence gene characteristics of Enterococcus species from pig farms. A total of 300 faecal samples were obtained from two pig farms in Benin City between February and July 2016. Standard culture-based and polymerase chain reaction (PCR) assay were adopted in the detection and characterization of the Enterococcus species. Antimicrobial susceptibility profile was determined using disc diffusion method. A total of 268 enterococci isolates were recovered from both farms investigated. In Farm A, 94/95 (99%) of E. faecalis isolates were resistant to clindamycin; while 23/25 (92%) of E. faecium isolates were resistant to clindamycin. In farm B, all E. faecalis isolates 119/119 (100%) were resistant to clindamycin; while 26/29 (90%) of E. faecium isolates were resistant to clindamycin. Virulence gene detected in the enterococci isolates includes aggregation ( asa 1) [Farm A ( E. faecalis 66%, E. faecium 76%), Farm B ( E. faecalis 71%, E. faecium 13%)] and others. Multidrug resistant profile of the isolates revealed that 17/95 (18%) of E. faecalis and 3/25 (12%) of E. faecium isolates from Farm A as well as, 16/119 (14%) of E. faecalis and 5/29 (17%) of E. faecium isolates from Farm B were resistant to CLI R, PEN R, ERY R, GEN R, TET R, MEM R, KAN R, and PTZ R . The high level of resistance observed in the study and their virulence gene signatures, calls for effective environmental monitoring to circumvent the environmental dissemination of resistant pathogenic clones. Thus environmental hygiene should be provided to food animals to prevent the proliferation and spread of resistant bacteria. Graphical abstract: Highlights: Multiple antibiotic resistances are problematic in human and zoonotic pathogens. E. faecalis and E. faecium were recovered from piggery farms. High level of resistance was observed in the Enterococcus species. Enterococcus species had genetic traits of virulent determinants. The capacity of Enterococcus species to genetically harbour virulence traits to enhance their pathogenicity is worrisome.