Green extracts from Coratina olive cultivar leaves: Antioxidant characterization and biological activity. (April 2017)

• Record Type: Journal Article
• Authors: Difonzo, Graziana; Russo, Annamaria; Trani, Antonio; Paradiso, Vito M.; Ranieri, Marianna; Pasqualone, Antonella; Summo, Carmine; Tamma, Grazia; Silletti, Roccangelo; Caponio, Francesco
• Is Part Of: Journal of functional foods. Volume 31(2017)
• Page Start: 63
• Page End: 70
• Publication Date: 2017-04
• Subjects: Olive leaves -- Antioxidant activity -- Biophenols -- Green extraction -- Reactive oxygen species; ABTS 2, 2′-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt -- BHA butylated hydroxyanisole -- BHT butylated hydroxytoluene -- CTR control -- DPPH 2, 2-diphenyl-1-picrylhydrazyl -- Eth ethanol -- F fresh -- FD freeze dried -- HD hot air-dried -- ROS reactive oxygen species -- TBHP tert-butylhydroperoxide -- TPC total phenol content -- TE Trolox equivalents -- TEAC Trolox equivalent antioxidant capacity -- Trolox (±)-6-hydroxy-2, 5, 7, 8-tetramethylchromane-2-carboxylic acid -- UHPLC-ESI-MS/MS ultra-high performance liquid chromatography electrospray ionization tandem mass spectrometry; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2017.01.039
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: The olive leaves represent a source of bioactive compounds. A green extraction method of biophenols from leave olives was set-up. Leaves extracts exhibit a good antioxidant ability. Abstract: Olive leaves and their extracts are associated with food preservation and health, and are used in folk medicine to treat several diseases, mainly because of their polyphenols. In this investigation polyphenols were extracted from olive leaves using green solvents and evaluated the antioxidant activity of the extracts. Polyphenols were extracted from fresh, freeze-dried, and hot air-dried leaves using either ethanol/water mixtures (70:30, 30:70, v/v) or water alone. Antioxidant activity was assessed in bronchial epithelial NCI-H292 cells by measuring reactive oxygen species (ROS) and in vegetable oil by measuring oxygen consumption. Results showed that extracts with a good antioxidant activity could be obtained when leaves were pre-treated by hot air-drying. The use of water alone as the extraction solvent gave results comparable to those obtained with ethanol/water (30:70, v/v). These extracts were particularly rich in oleuropein, and had anti-ROS effects in NCI-H292 cells and antioxidant activity in vegetable oil.