Strong anti-Epstein Barr virus (EBV) or cytomegalovirus (CMV) cellular immune responses predict survival and a favourable response to anti-tuberculosis therapy. (March 2017)

• Record Type: Journal Article
• Authors: Nagu, Tumaini; Aboud, Said; Rao, Martin; Matee, Mecky; Axelsson, Rebecca; Valentini, Davide; Mugusi, Ferdinand; Zumla, Alimuddin; Maeurer, Markus
• Is Part Of: International journal of infectious diseases. Volume 56(2017:Mar.)
• Page Start: 136
• Page End: 139
• Publication Date: 2017-03
• Subjects: pulmonary tuberculosis -- EBV -- CMV -- cellular immune response -- IFN-γ -- survival -- TB therapy,; Communicable diseases -- Periodicals; Communicable Diseases -- Periodicals; Communicable diseases; Periodicals; Electronic journals; 616.9
• DOI: 10.1016/j.ijid.2017.01.022
• Languages: English
• ISSNs: 1201-9712

Abstract

Highlights: CMV, EBV and ESAT-6 – specific IFN-γ responses in patients with pulmonary TB at diagnosis correlate with cure and survival during anti-TB therapy. CMV/EBV responses maybe good biomarkers to identify patients at high risk to succumb to TB disease. CVM/EBV-vectored vaccines are recommended for future TB vaccine development. CMV/EBV-specific cellular immune responses will be valuable in identifying time points for Host-Directed Therapies (HDTs). Abstract: Background: Intact immune responses to cytomegalovirus (CMV) and Epstein-Barr virus (EBV) represent a biologically and clinically relevant correlate of 'immunological fitness' in humans. However, there is a lack of knowledge concerning anti-EBV or anti-CMV responses in patients with pulmonary tuberculosis (TB), in whom aberrant immune responses may promote progression of clinical disease. Methods: Venous blood samples were obtained at the time of (sputum smear positive) pulmonary TB diagnosis. A whole blood assay was performed by exposing PBMCs (peripheral blood mononuclear cells) to a panel of infectious antigens, including CMV, EBV and mycobacterial proteins. Cell culture supernatants were collected after seven days and interferon gamma (IFN-γ) was measured using a sandwich ELISA. Patients received standard first line anti-tuberculosis rifampicin (R)/isoniazid (H)/ethambutol (E)/pyrazinamide (Z) for two months followed by RH for four months. Results: PBMCs from cured patients (after treatment completion) exhibited significantly stronger IFN-γ responses to CMV (p=0.035), EBV (p=0.006) or Mycobacterium tuberculosis ESAT-6 (p = 0.043) at the time of diagnosis as compared to patients who succumbed to TB during treatment. IFN-γ responses to other viral (H5N1, HSV-1) as well as other mycobacterial (Ag85A, Rv2958c, Rv0447c) antigens were not found to be significantly different among patients who were cured or those who succumbed to TB. Conclusions: Increased cellular immune responses to CMV and EBV antigens at the time of diagnosis of pulmonary tuberculosis are associated with increased survival after a standard six months anti-TB therapy. CVM and EBV antigens may represent "intrinsic markers for immune fitness" and guide improved TB therapies including host-directed therapies.