Anti-inflammatory effect of strawberry extract against LPS-induced stress in RAW 264.7 macrophages. (April 2017)

• Record Type: Journal Article
• Authors: Gasparrini, Massimiliano; Forbes-Hernandez, Tamara Y.; Giampieri, Francesca; Afrin, Sadia; Alvarez-Suarez, Josè M.; Mazzoni, Luca; Mezzetti, Bruno; Quiles, Josè L.; Battino, Maurizio
• Is Part Of: Food and chemical toxicology. Volume 102(2017)
• Page Start: 1
• Page End: 10
• Publication Date: 2017-04
• Subjects: Strawberry -- Phenolic compounds -- LPS -- Inflammation -- ROS -- Prevention; 2, 4 DNP 2, 4-Dinitrophenol -- ACYs anthocyanins -- ARE antioxidant responsive element -- CDNB 1-chloro-2, 4-dinitro benzene -- DMEM Dulbecco's Modified Eagle Medium -- DPPH 2, 2-DiPhenyl-1-PicrylHydrazyl free radical method -- ETC electron transport chain -- FRAP Ferric Reducing Antioxidant Power -- FW fresh weight -- GSH reduced glutathione -- GPx glutathione peroxidase -- GR glutathione reductase -- GST glutathione transferase -- HO-1 heme oxygenase 1 -- IL-1β interleukin 1-beta -- IL-6 interlukin 6 -- IL-10 interlukin 10 -- iNOS inducible nitric oxide synthase -- LPS lipopolysaccharide -- NF-kB Nuclear factor kappa-light-chain-enhancer of activated B cells -- NO nitric oxide -- NO2− nitrite -- Nrf2 nuclear factor E2-related factor 2 -- OCR oxygen consumption rate -- OGG1 8-Oxoguanine glycosylase -- pAMPK phosphorylated 5′ AMP-activated protein kinase -- pIκBα phosphorylated nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha -- PBS phosphate-buffered saline solution -- PGC1α peroxisome proliferator-activated receptor c coactivator 1 alpha -- ROS reactive oxygen species -- SD standard deviation -- SIRT1 sirtuin 1 -- SOD superoxide dismutase -- TAC total antioxidant capacity -- TBARS thiobarbituric acid-reactive substances -- TEAC Trolox Equivalent Antioxidant Capacity -- TFC total flavonoid content -- TNF-α tumor necrosis factor alpha -- TPC total phenolic content -- vit C vitamin C; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2017.01.018
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: A common denominator in the pathogenesis of most chronic inflammatory diseases is the involvement of oxidative stress, related to ROS production by all aerobic organisms. Dietary antioxidants from plant foods represent an efficient strategy to counteract this condition. The aim of the present study was to evaluate the protective effects of strawberry extracts on inflammatory status induced by E. Coli LPS on RAW 264.7 macrophages by measuring the main oxidative and inflammatory biomarkers and investigating the molecular pathways involved. Strawberry pre-treatment efficiently counteracted LPS-induced oxidative stress reducing the amount of ROS and nitrite production, stimulating endogenous antioxidant enzyme activities and enhancing protection against lipid, protein and DNA damage ( P < 0.05). Strawberry pre-treatment exerted these protective effects primarily through the activation of the Nrf2 pathway, which is markedly AMPK-dependent and also by the modulation of the NF-kB signalling pathway. Finally, an improvement in mitochondria functionality was also detected. The results obtained in this work highlight the health benefit of strawberries against inflammatory and oxidative stress in LPS-stimulated RAW 264.7 macrophages, investigating for the first time the possible involved molecular mechanisms. Graphical abstract: Highlights: Strawberry treatment counteracted LPS-induced inflammation in RAW 264.7 macrophages. Strawberry treatment improved antioxidant defense. Strawberry treatment reduced inflammatory cytokines production.