Anti-inflammatory effect of Capuli cherry against LPS-induced cytotoxic damage in RAW 264.7 macrophages. (April 2017)

• Record Type: Journal Article
• Authors: Alvarez-Suarez, José M.; Carrillo-Perdomo, Estefanía; Aller, Angel; Giampieri, Francesca; Gasparrini, Massimiliano; González-Pérez, Lien; Beltrán-Ayala, Pablo; Battino, Maurizio
• Is Part Of: Food and chemical toxicology. Volume 102(2017)
• Page Start: 46
• Page End: 52
• Publication Date: 2017-04
• Subjects: Prunus serotina -- Capuli cherry -- Antioxidant capacity -- Oxidative stress -- Inflammation; NO nitric oxide -- TNF-α tumor necrosis factor-α -- DW dry weight -- TPC total phenolic content -- GAE galic acid equivalents -- TFC total flavonoid content -- Cateq catechin equivalents -- ACYs total anthocyanins content -- Pg-3-gluc pelargonidin 3-glucoside -- PgEq Pg-3-gluc equivalents -- TAC total antioxidant capacity -- TEq trolox equivalents -- TEAC trolox equivalent antioxidant capacity -- FRAP ferric reducing antioxidant power -- O2– superoxide radical -- αTE α-tocopherol equivalents -- AscEq ascorbate equivalents -- CCE capuli crude extract -- DMSO dimethyl sulfoxide -- LPS lipopolysaccharide -- ROS reactive oxygen species -- DCFH 2′-7′-dichlorofluorescin diacetate -- CAT catalase -- SOD superoxide dismutase -- TBARS thiobarbituric acid reactive substances -- ABTS+ [2, 2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)] radical cation -- Nrf2 nuclear factor-erythroid 2-related factor 2 -- AMPK 5′ adenosine monophosphate-activated protein kinase -- AMPK/Nrf2/ARE (Kelch ECH associating protein 1/NF-E2-related factor 2/antioxidant responsive elements) signalling pathway -- IL1β interleukin 1 beta; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2017.01.024
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Capuli cherry ( Prunus serotina Ehr. subsp. capuli (Cav.) McVaugh) fruits from the inter-Andean region of Ecuador were analysed to determine their bioactive compounds content, total antioxidant capacity, radical scavenging activity and their anti-inflammatory and protective effects against the cytotoxic damage mediated by lipopolysaccharide (LPS) in RAW 264.7 macrophages. Capuli fruits proved to be a natural source of bioactive compounds such as anthocyanins, vitamin C and β -carotene as well as to present an important total antioxidant capacity and radical scavenging activities. RAW 264.7 macrophages were incubated with different concentration of Capuli crude extract and subsequently activated by LPS to determine the markers related to oxidative damage and the proinflammatory cytokine production. The markers of oxidative damage, nitrite levels, the interleukin 1β messenger RNA levels and the tumor necrosis factor α mRNA levels and secretion were significantly decreased after the pre-incubated with Capuli extract and subsequently stimulated with LPS. In summary, Capuli extract attenuated the LPS-induced damage in RAW 264.7 macrophages due to its antioxidant and anti-inflammatory properties, showing that Capuli fruits may represent a relevant source of bioactive compounds with promising benefits for human health. Highlights: Capuli fruits represent a relevant source of bioactive compounds. Capuli crude extract shows important antioxidant properties. Capuli crude extract suppressing the expression and secretion of proinflammatory mediators. Capuli crude extract attenuated the LPS-induced cytotoxic damage in RAW 264.7 cells.