Super RLuc8-sFv; a new luciferase-labeled probe for detection of human CD4+ cells. Issue 3 (23rd January 2017)

• Record Type: Journal Article
• Authors: Safavi, A.; Emamzadeh, R.; Nazari, M.; Ehsani, M.; Zarkesh-Esfahani, S. H.; Rahgozar, S.
• Is Part Of: Molecular bioSystems. Volume 13:Issue 3(2017)
• Page Start: 470
• Page End: 475
• Publication Date: 2017-01-23
• Subjects: Molecular biology -- Periodicals; Biochemistry -- Periodicals; 571.7405
• DOI: 10.1039/c6mb00652c
• Languages: English
• ISSNs: 1742-206X

Abstract

Abstract : A probe has been designed, produced and assayed for the detection of T-helper cells. The sFv fragment fused to the C-terminus of super Renilla luciferase 8 (Super RLuc8) and the probe successfully used for detection of human CD4+ cells using luminometer. Abstract : The human immunodeficiency virus (HIV) destroys CD4+ lymphocytes and monitoring these cells is one of the best techniques for studying HIV infection. In the present study a novel bioluminescent probe, super RLuc8-sFv, is developed in order to detect human CD4+ cells by fusion of an anti-human CD4 sFv to the C-terminus of super RLuc8. The results indicate that the probe can bind to CD4+ cells via its sFv domain; also it emits visible light through its signalling domain. Super RLuc8-sFv provides a new gateway for detection of human CD4+ cells using luminometric-based assays and may reduce the difficulties involved in, and the cost of, HIV-related diagnostic tests.