Preliminary neutron diffraction analysis of challenging human manganese superoxide dismutase crystals. Issue 4 (1st April 2017)

• Record Type: Journal Article
• Authors: Azadmanesh, Jahaun; Trickel, Scott R.; Weiss, Kevin L.; Coates, Leighton; Borgstahl, Gloria E. O.
• Is Part Of: Acta crystallographica. Volume 73:Issue 4(2017:Apr.)
• Page Start: 235
• Page End: 240
• Publication Date: 2017-04-01
• Subjects: manganese superoxide dismutase -- neutron diffraction -- perdeuteration -- human -- large unit cell; Crystallography -- Periodicals; Crystals -- Periodicals; 548
• DOI: 10.1107/S2053230X17003508
• Languages: English
• ISSNs: 2053-230X

Abstract

Abstract : The growth of crystals of perdeuterated human manganese superoxide dismutase with a unit‐cell edge of 240 Å was optimized for neutron data collection. Data were collected to 2.30 Å resolution from a 0.26 mm 3 crystal. This is the crystal with the largest unit‐cell edge from which data have been collected via neutron diffraction to sufficient resolution where hydrogen positions can be observed. Abstract : Superoxide dismutases (SODs) are enzymes that protect against oxidative stress by dismutation of superoxide into oxygen and hydrogen peroxide through cyclic reduction and oxidation of the active‐site metal. The complete enzymatic mechanisms of SODs are unknown since data on the positions of hydrogen are limited. Here, methods are presented for large crystal growth and neutron data collection of human manganese SOD (MnSOD) using perdeuteration and the MaNDi beamline at Oak Ridge National Laboratory. The crystal from which the human MnSOD data set was obtained is the crystal with the largest unit‐cell edge (240 Å) from which data have been collected via neutron diffraction to sufficient resolution (2.30 Å) where hydrogen positions can be observed.