Antiepileptic effect of fisetin in iron-induced experimental model of traumatic epilepsy in rats in the light of electrophysiological, biochemical, and behavioral observations. (21st April 2017)

• Record Type: Journal Article
• Authors: Das, Jharana; Singh, Rameshwar; Sharma, Deepak
• Is Part Of: Nutritional neuroscience. Volume 20:Number 4(2017)
• Page Start: 255
• Page End: 264
• Publication Date: 2017-04-21
• Subjects: Fisetin -- Epilepsy -- Oxidative stress -- Na+ -- K+-ATPase -- Lipid peroxidation -- Morris water maze -- Open-field test; Neuropharmacology -- Periodicals; Diet -- Periodicals; Diet therapy -- Periodicals; Nutrition -- Periodicals; 615.78
• DOI: 10.1080/1028415X.2016.1183342
• Languages: English
• ISSNs: 1028-415X

Abstract

Abstract : Background : Traumatic epilepsy is defined by episodes of recurring seizures secondary to severe brain injury. Though drugs are found effective to control seizures, their long-term use have been observed to increase reactive oxygen species in animals. Flavonoid fisetin, a natural bioactive phytonutrient reported to exert anticonvulsive effect in experimental seizure models. But, trauma-induced seizures could not be prevented by anticonvulsants was reported in some clinical studies. Objective : To study the effect of fisetin on epileptiform electrographic activity in iron-induced traumatic epilepsy and also the probable reason behind the effect in rats. Methods : Fisetin pretreatment (20 mg/kg body wt., p.o.) of rats for 12 weeks were chosen followed by injecting iron (5 µl, 100 mM) stereotaxically to generate iron-induced epilepsy. Experimental design include electrophysiological study (electroencephalograph in correlation with multiple unit activity (MUA) in the cortex and CA1 subfield of the hippocampus; spectral analysis of seizure and seizure-associated behavioral study (Morris water maze for spatial learning, open-field test for anxiety) and biochemical study (lipid peroxidation, Na +, K + -ATPase activity) in both the cortex and the hippocampus. Results : Fisetin pretreatment was found to prevent the development of iron-induced electrical seizure and decrease the corresponding MUA in the cortex (*P˂0.05) as well as in the hippocampus (*** P ˂0.001). Fisetin pretreatment decreased the lipid peroxides (* P ˂0.05) and retained the Na +, K + -ATPase activity (* P ˂0.05) which was found altered in the epileptic animals and also found to attenuate the seizure-associated cognitive dysfunctions. Conclusion : This study demonstrated the antiepileptic action of fisetin in iron-induced model of epileptic rats by inhibiting oxidative stress.