A flow-through holed PDMS membrane as a reusable microarray spotter for biomedical assays. Issue 1 (31st October 2014)

• Record Type: Journal Article
• Authors: Lamberti, A.; Angelini, A.; Ricciardi, S.; Frascella, F.
• Is Part Of: Lab on a chip. Volume 15:Issue 1(2015)
• Page Start: 67
• Page End: 71
• Publication Date: 2014-10-31
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/c4lc01027b
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : A holed-designed PDMS membrane is proposed as an innovative microarray spotter exploiting a vacuum procedure and verifying a good level of reproducibility of spotting fluorescent proteins over large areas. Abstract : We propose the exploitation of a holed-designed poly(dimethyl)siloxane (PDMS) membrane as an innovative microarray spotter. The membrane is fabricated by a simple technological approach and can be reused several times. A good level of reproducibility is found upon spotting fluorescent proteins at different concentrations over large areas.