An integrated centrifugo-opto-microfluidic platform for arraying, analysis, identification and manipulation of individual cells. Issue 2 (12th November 2014)

• Record Type: Journal Article
• Authors: Burger, R.; Kurzbuch, D.; Gorkin, R.; Kijanka, G.; Glynn, M.; McDonagh, C.; Ducrée, J.
• Is Part Of: Lab on a chip. Volume 15:Issue 2(2015)
• Page Start: 378
• Page End: 381
• Publication Date: 2014-11-12
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/c4lc01002g
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : In this work we present a centrifugal microfluidic system enabling highly efficient collective trapping and alignment of particles such as microbeads and cells, their multi-colour fluorescent detection and subsequent manipulation by optical tweezers. Abstract : In this work we present a centrifugal microfluidic system enabling highly efficient collective trapping and alignment of particles such as microbeads and cells, their multi-colour fluorescent detection and subsequent manipulation by optical tweezers. We demonstrate array-based capture and imaging followed by "cherry-picking" of individual particles, first for fluorescently labelled polystyrene (PS) beads and then for cells. Different cell lines are discriminated based on intracellular as well as surface-based markers.