A microsystem integrating photodegradable hydrogel microstructures and reconfigurable microfluidics for single-cell analysis and retrieval. Issue 3 (25th November 2014)

• Record Type: Journal Article
• Authors: Son, Kyung Jin; Shin, Dong-Sik; Kwa, Timothy; You, Jungmok; Gao, Yandong; Revzin, Alexander
• Is Part Of: Lab on a chip. Volume 15:Issue 3(2015)
• Page Start: 637
• Page End: 641
• Publication Date: 2014-11-25
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/c4lc00884g
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : We developed a micropatterned photodegradable hydrogel array integrated with reconfigurable microfluidics to enable cell secretion analysis and cell retrieval at the single-cell level. Abstract : We developed a micropatterned photodegradable hydrogel array integrated with reconfigurable microfluidics to enable cell secretion analysis and cell retrieval at the single-cell level. The activity of protease molecules secreted from single cells was monitored using FRET peptides entrapped inside microfabricated compartments. Antibody-modified gel islands tethering cells to the surface could be degraded by UV exposure to release specific single cells of interest.