Large area CMOS bio-pixel array for compact high sensitive multiplex biosensing. Issue 3 (10th December 2014)

• Record Type: Journal Article
• Authors: Sandeau, Laure; Vuillaume, Cassandre; Contié, Sylvain; Grinenval, Eva; Belloni, Federico; Rigneault, Hervé; Owens, Roisin M.; Fournet, Margaret Brennan
• Is Part Of: Lab on a chip. Volume 15:Issue 3(2015)
• Page Start: 877
• Page End: 881
• Publication Date: 2014-12-10
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/c4lc01025f
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : Individual CMOS Bio-pixels with triplicate assay for cytokines TNFα, IL8 and IFN. Expansion shows a schematic of light emission confinement. Abstract : A novel CMOS bio-pixel array which integrates assay substrate and assay readout is demonstrated for multiplex and multireplicate detection of a triplicate of cytokines with single digit pg ml −1 sensitivities. Uniquely designed large area bio-pixels enable individual assays to be dedicated to and addressed by single pixels. A capability to simultaneously measure a large number of targets is provided by the 128 available pixels. Chemiluminescent assays are carried out directly on the pixel surface which also detects the emitted chemiluminescent photons, facilitating a highly compact sensor and reader format. The high sensitivity of the bio-pixel array is enabled by the high refractive index of silicon based pixels. This in turn generates a strong supercritical angle luminescence response significantly increasing the efficiency of the photon collection over conventional farfield modalities.