Co-localization of proteins with defined sequential order and controlled stoichiometric ratio on magnetic nanoparticles. Issue 13 (20th March 2017)

• Record Type: Journal Article
• Authors: Yue, Yi; Lu, Yang-Yang; Li, Mei; Zhang, Zi-Jian; Tan, Tian-Wei; Fan, Li-Hai
• Is Part Of: Nanoscale. Volume 9:Issue 13(2017)
• Page Start: 4397
• Page End: 4400
• Publication Date: 2017-03-20
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/c7nr00557a
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : Here we reported a method for controlled co-immobilization of multiple proteins on magnetic nanoparticles by using cohesin–dockerin and His tag-nickel(ii ) interactions. Abstract : Co-immobilization of enzymes used in cascade reactions is important for improving the overall catalytic efficiency. In this work, we employed scaffoldins as a bridge and succeeded in a highly-ordered co-localization of multiple proteins on magnetic nanoparticles with a loading capacity of ∼0.831 μmol g −1 supports.