Rapid detection of Hendra virus antibodies: an integrated device with nanoparticle assay and chaotic micromixing. Issue 1 (6th December 2016)

• Record Type: Journal Article
• Authors: Petkovic, K.; Metcalfe, G.; Chen, H.; Gao, Y.; Best, M.; Lester, D.; Zhu, Y.
• Is Part Of: Lab on a chip. Volume 17:Issue 1(2017)
• Page Start: 169
• Page End: 177
• Publication Date: 2016-12-06
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/c6lc01263a
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : An integrated device with nanoparticle assay and chaotic micromixing for rapid detection of Hendra virus antibodies. Abstract : Current diagnosis of infectious diseases such as Hendra virus (HeV) relies mostly on laboratory-based tests. There is an urgent demand for rapid diagnosis technology to detect and identify these diseases in humans and animals so that disease spread can be controlled. In this study, an integrated lab-on-a-chip device using a magnetic nanoparticle immunoassay is developed. The key features of the device are the chaotic fluid mixing, achieved by magnetically driven motion of nanoparticles with the optimal mixing protocol developed using chaotic transport theory, and the automatic liquid handling system for loading reagents and samples. The device has been demonstrated to detect Hendra virus antibodies in dilute horse serum samples within a short time of 15 minutes and the limit of detection is about 0.48 ng ml −1 . The device platform can potentially be used for field detection of viruses and other biological and chemical substances.