Histologic changes of mesenchymal stem cell repair of tympanic membrane perforation. (3rd April 2017)

• Record Type: Journal Article
• Authors: Goncalves, Stefania; Bas, Esperanza; Langston, Michael; Grobman, Ariel; Goldstein, Bradley J.; Angeli, Simon
• Is Part Of: Acta oto-laryngologica. Volume 137:Number 4(2017)
• Page Start: 411
• Page End: 416
• Publication Date: 2017-04-03
• Subjects: Tympanic membrane -- stem cells -- chronic tympanic membrane perforations -- wound healing; Otolaryngology -- Periodicals; Ear -- Diseases -- Periodicals; Throat -- Diseases -- Periodicals; Otolaryngology -- Electronic Resources; Otorhinolaryngologic Diseases; 617.8
• DOI: 10.1080/00016489.2016.1261411
• Languages: English
• ISSNs: 0001-6489

Abstract

Abstract: Conclusion: Mesenchymal stem-cells are good candidates for cell-therapy of chronic tympanic membranes perforations. Objectives: To determine the effects of cell-based therapy in tympanic membrane perforations. Methods: Young C57BL/6 mice were anesthetized with intraperitoneal administration of ketamine and xylazine and randomly divided into three groups ( n = 4 ears/group) that underwent bilateral sub-total pars tensa perforations of equal sizes using a sterile 27-gauge needle under a surgical microscope. Six-to-eight hours after injury, one group of mice did not receive treatment (acute perforation control), and the last two groups were treated with BM-MSCs embedded within HA scaffolds previously soaked in PBS to rinse culture media residues to avoid confounders and were euthanized 1 or 2 weeks after treatment. Results: Untreated tympanic membrane perforations developed a hyper-cellular infiltrate surrounding the injury site, while BM-MSC treated eardrums showed a reduced inflammatory response after the first week and a restoration of the trilaminar configuration 2 weeks after treatment, mimicking a normal tympanic membrane.