Flexible Microgap Electrodes by Direct Inkjet Printing for Biosensing Application. Issue 3 (15th February 2017)

• Record Type: Journal Article
• Authors: Adly, Nouran; Feng, Lingyan; Krause, Kay J.; Mayer, Dirk; Yakushenko, Alexey; Offenhäusser, Andreas; Wolfrum, Bernhard
• Is Part Of: Advanced biosystems. Volume 1 :Issue 3 (2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-02-15
• Subjects: amplification -- biosensor -- inkjet -- microgap -- redox‐cycling; Biological systems -- Periodicals; Biotechnology -- Periodicals; Bioengineering -- Periodicals; Biomedical engineering -- Periodicals; Biological Science Disciplines; Periodicals; Periodicals; 660.6
• DOI: 10.1002/adbi.201600016
• Languages: English
• ISSNs: 2366-7478

Abstract

Abstract : A rapid fabrication method of microgap electrodes using inkjet printing is described. In this approach, the lateral spacing between two printed electrode lines is precisely controlled down to 1 µm without any surface modification or substrate patterning. The strong confinement, well below typical resolution of inkjet printing, relies on complete solvent evaporation between the printing of adjacent electrode structures, which is achieved by controlling the printing speed and temperature profiles. The feasibility of this method is demonstrated by writing electrode structures with two distinct inks, based on carbon and silver nanoparticles, with comparable results. As an application proof‐of‐principle, arrays of microgap electrodes are fabricated using a carbon nanoparticle ink for electrochemical detection based on redox‐cycling, a technique in which the sensitivity of the device depends on the distance between the two electrodes. The redox‐cycling amplification of electrochemical signals is demonstrated and it is shown that the printed microgap device can be used as an electrochemical biosensor for the determination of human immunodeficiency virus (HIV)‐related single‐stranded DNA. This work presents a promising new approach for fabricating low‐cost and label‐free redox‐cycling biosensors using all‐inkjet‐printed electrodes. Abstract : This study reports the fabrication of electrochemical biosensors using high‐resolution ink‐jet printing technology. Printed microgap devices amplify the electrochemical current of a redox tracer, which can be blocked by interactions with specific target molecules. The sensors are used for the detection of single‐stranded DNA related to the human immunodeficiency virus.