Arsenic affects on cerebellar development of mice. (27th September 2013)

• Record Type: Journal Article
• Authors: Ding, Xiaohui; Su, Qiuxiang; Jiang, Min; Xie, Hui; Cong, Jing; Wang, Luan
• Is Part Of: Toxicology mechanisms and methods. Volume 23:Number 9(2013)
• Page Start: 672
• Page End: 677
• Publication Date: 2013-09-27
• Subjects: 5′-bromo-2′-deoxyuridine (BrdU) -- 8-oxo-7 -- 8-dihydro-2′-deoxyguanosine (8-oxodG) -- arsenic -- mitosis -- mice; Analytical toxicology -- Periodicals; Toxicology -- Periodicals; Toxicology -- Methodology -- Periodicals; 615.907
• DOI: 10.3109/15376516.2013.840347
• Languages: English
• ISSNs: 1537-6516

Abstract

Abstract: To evaluate arsenic affects on the development of cerebellum, the mother mice received drinking water containing arsenic trioxide (As2 O3) 4 ppm during gestation and lactation period. The cytogenesis was observed by immunohistochemical technique using 5′-bromo-2′-deoxyuridine (BrdU) antibody. To characterize the arsenic neurotoxicity, 8-oxo-7, 8-dihydro -2′-deoxyguanosine (8-oxodG), an oxidative DNA lesion marker, and 8-nitroguanine, a nitrative DNA lesion marker were used to verify DNA damage. Arsenic-exposed litters showed neuron necrosis. The mitosis of granule cells decreased dramatically in arsenic-exposed mice as compared with arsenic unexposed mice. 8-OxodG was formed in neurons of all the layers, especially in the granular layer in cerebellum of arsenic-exposed mice. There is no significant difference, however, in the expression of 8-nitroguanine between arsenic-exposed and -unexposed mice. These results indicate that arsenic can disturb the mitosis of granule cells and interfere with the normal development of mice cerebellum. Arsenic-induced pathological changes in vivo may be caused by oxidative DNA damage other than nitrative DNA damage.