5-Azacytidine inhibits proliferation and induces apoptosis of mouse bone marrow-derived mesenchymal stem cells. (11th October 2013)

• Record Type: Journal Article
• Authors: Zhang, Fenxi; Wu, Junfang; Lu, Ming; Wang, Huaibin; Feng, Huigen
• Is Part Of: Toxin reviews. Volume 32:Number 4(2013)
• Page Start: 55
• Page End: 59
• Publication Date: 2013-10-11
• Subjects: 5-Azacytidine -- apoptosis -- bone marrow-derived mesenchymal stem cells -- Caspase-3 -- proliferation; Toxicology -- Periodicals; Toxins -- Periodicals; 615.905
• DOI: 10.3109/15569543.2013.846382
• Languages: English
• ISSNs: 1556-9543

Abstract

Abstract: In this study, we investigated the effect of 5-azacytidine on proliferation and apoptosis of bmMSCs. After exposure to different concentrations of 5-azacytindine, bmMSC proliferation and apoptosis were measured by MTT assay, Caspase-3 staining and western blotting. Our results show that 1 μM 5-azacytidine has no significant effect on viability of bmMSCs; however, 5–20 μM 5-azacytindine significantly inhibits bmMSC proliferation and expression of phospho-Akt; 10 and 20 μM 5-azacytidine markedly increases bmMSC apoptosis and expression of Caspase-3, Annexin V and Bax, and decreases expression of Bcl2. Our findings indicate that 5-azacytidine at the commonly used doses has toxicity to bmMSCs.