Gold nanoparticle–mesoporous silica sheet composites with enhanced antibody adsorption capacity. (25th March 2015)

• Record Type: Journal Article
• Authors: Nakanishi, Kazuma; Tomita, Masahiro; Masuda, Yoshitake; Kato, Katsuya
• Is Part Of: New journal of chemistry. Volume 39:Number 5(2015:May)
• Page Start: 4070
• Page End: 4077
• Publication Date: 2015-03-25
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/c5nj00033e
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : For the enhancement of antibody adsorption capacity, gold nanoparticle–MPS sheet composites were prepared. The dispersed gold nanoparticles were loaded onto an MPS sheet via deposition–precipitation using HAuCl4 . Abstract : A notably high-capacity antibody adsorbent was prepared using dispersed gold nanoparticles immobilised on a mesoporous silica sheet. The mesoporous silica sheet (MPS sheet) was prepared via a dual-templating method using tetraethoxysilane and 3-aminopropyltriethoxysilane as the silica framework materials. Gold nanoparticles were loaded onto the amino-functionalised surface of the MPS sheet via deposition–precipitation using HAuCl4 as the Au colloid precursor and then crystallised via calcination. The properties of the gold nanoparticle–silica composite and the dispersion of the nanoparticles were characterised using transmission electron microscopy, energy-dispersive X-ray spectroscopy and X-ray diffraction analysis. The surface characteristics of gold nanoparticle composite materials calcined at different temperatures were also evaluated using Fourier transform-infrared spectroscopy, X-ray photoelectron spectroscopy and thermogravimetry data. The silica–gold nanoparticle composite sheet crystallised at 550 °C (sheet-Au-550) was mesoporous in character (pore diameter = 3.8 nm) with a BET surface area of 258.0 m 2 g −1 . With respect to antibody adsorption, the sheet materials had a relatively higher capacity than the typical cylindrical mesoporous silica, and sheet-Au-550 exhibited a strong performance (maximum adsorption quantity Q Max = 0.26 mg IgG per mg carrier). In addition, the IgG adsorption equilibrium for sheet-Au-550 fits the Langmuir isotherm model better than the Freundlich plot model. These results will be very useful for the design of adsorption materials for biomolecules, such as antibodies.