Biological activity of peptide‐conjugated polyion complex matrices consisting of alginate and chitosan. Issue 1 (January 2017) - Fujimori, Chikara
Abstract: Peptide‐conjugated polysaccharide matrices using bioactive laminin‐derived peptides are useful biomaterials for tissue and cell engineering. Here, we demonstrate an easy handling preparation method for peptide‐polysaccharide matrices using polyion complex with both alginate and chitosan. First, aldehyde‐alginate was synthesized by oxidization of alginate using NaIO4, and then, reacted with Cys‐peptides. Next, the peptide‐alginate solution was added to a chitosan‐coated plate, and the peptide‐polyion complex matrices (peptide‐PCMs) were prepared. The peptide‐PCMs using an integrin αvβ3‐binding peptide (A99a: ALRGDN, mouse laminin α1 chain 1145‐1150) and an integrin α2β1‐binding peptide (EF1XmR: RLQLQEGRLHFXFD, X = Nle, mouse laminin α1 chain 2751‐2763) showed strong cell attachment activity in a dose‐dependent manner. When we examined the effect of various spacers on the biological activity of A99a‐PCM, hydrophobic and long spacers enhanced the cell attachment activity. Further, the A99a‐PCM with the spacers strongly promoted neurite outgrowth. The polyion complex method is an easy way to obtain insolubilized matrix and is widely applicable for various polysaccharides. The peptide‐PCM is useful as a biomaterial for cell and tissue engineering. Abstract :