Drug- not carrier-dependent haematological and biochemical changes in a repeated dose study of cyclosporine encapsulated polyester nano- and micro-particles: Size does not matter. (1st April 2015)

• Record Type: Journal Article
• Authors: Venkatpurwar, V.P.; Rhodes, S.; Oien, K.A.; Elliott, M.A.; Tekwe, C.D.; Jørgensen, H.G.; Kumar, M.N.V. Ravi
• Is Part Of: Toxicology. Volume 330(2015)
• Page Start: 9
• Page End: 18
• Publication Date: 2015-04-01
• Subjects: CsA cyclosporine -- PLGA poly(lactide-co-glycolide) -- nCsA CsA encapsulated nanoparticles -- mCsA CsA encapsulated microparticles -- SD Sprague Dawley -- CPC eruloplasmin -- LDL low-density lipoprotein -- BUN blood urea nitrogen -- PC plasma creatinine -- FBG fibrinogen -- CRP C-reactive protein -- SOD superoxide dismutase -- CAT catalases -- Hb haemoglobin -- RBC red blood corpuscles count -- WBC white blood corpuscles count -- DLC differential leukocyte count -- PE phycoerythrin -- HSPC haemopoietic stem/progenitor cells -- FSC forward scatter properties -- SSC side scatter properties -- CD glomerular capillary tuft -- BD Bowman's capsule; Biodegradable -- Cyclosporine -- Microparticles -- Nanoparticles -- Nanotoxicology -- Oral -- Polyester -- Toxicity; Toxicology -- Periodicals; Chemicals -- Physiological effect -- Periodicals; 615.9005
• DOI: 10.1016/j.tox.2015.01.017
• Languages: English
• ISSNs: 0300-483X

Abstract

Highlights: The particulate delivery allows an increase in dose range without accrual of toxicities. The altered haematological and biochemical changes are drug, but not particle dependent. PLGA nano/microparticles are safe on subacute peroral dosing over 28 days. Nano-toxicology, drug needs to be considered. Abstract: Biodegradable nanoparticles are being considered more often as drug carriers to address pharmacokinetic/pharmacodynamic issues, yet nano-product safety has not been systematically proven. In this study, haematological, biochemical and histological parameters were examined on 28 day daily dosing of rats with nano- or micro-particle encapsulated cyclosporine (CsA) to confirm if any changes observed were drug or carrier dependent. CsA encapsulated poly(lactide-co-glycolide) [PLGA] nano- (nCsA) and micro-particles (mCsA) were prepared by emulsion techniques. CsA (15, 30, 45 mg/kg) were administered by oral gavage to Sprague Dawley (SD) rats over 28 days. Haematological and biochemical metrics were followed with tissue histology performed on sacrifice. Whether presented as nCsA or mCsA, 45 mg/kg dose caused significant loss of body weight and lowered food consumption compared to untreated control. Across the doses, both nCsA and mCsA produce significant decreases in lymphocyte numbers compared to controls, commensurate with the proprietary product, Neoral ® 15. Dosing with nCsA showed higher serum drug levels than mCsA presumably owing to the smaller particle size facilitating absorption. The treatment had no noticeable effects on inflammatory/oxidative stress markers or antioxidant enzyme levels, except an increase in ceruloplasmin (CP) levels for high dose nCsA/mCsA group. Further, only subtle, sub-lethal changes were observed in histology of nCsA/mCsA treated rat organs. Blank (drug-free) particles did not induce changes in the parameters studied. Therefore, it is extremely important that the encapsulated drug in the nano-products is considered when safety of the overall product is assessed rather than relying on just the particle size. This study has addressed some concerns surrounding particulate drug delivery, demonstrating safe delivery of CsA whilst achieving augmented serum concentrations.