Determining tissue origin of circulating epithelial cells (CEC) in patients with differentiated thyroid cancer by real-time PCR using thyroid mRNA probes. Issue 2 (28th January 2015)

• Record Type: Journal Article
• Authors: Sorg, Sarah; Pachmann, Katharina; Brede-Hekimian, Katya; Freesmeyer, Martin; Winkens, Thomas
• Is Part Of: Cancer letters. Volume 356:Issue 2(2015)Part B
• Page Start: 491
• Page End: 495
• Publication Date: 2015-01-28
• Subjects: cDNA complementary deoxyribonucleic acid -- CEC circulating epithelial cells -- DTC differentiated thyroid cancer -- EpCAM epithelial cell adhesion molecule -- FITC fluoroisothiocyanate -- GAPDH glyceraldehyde 3-phosphate dehydrogenase -- mRNA messenger ribonucleic acid -- NIS sodium–iodide symporter -- qRT-PCR quantitative real-time PCR -- sTg serum thyroglobulin -- TPO thyroperoxidase -- TSH thyroid stimulating hormone -- TSH-R TSH-receptor -- TTF1 Thyroid-transcription-factor 1; EpCAM -- Circulating tumour cells -- CTC -- mRNA -- Tg -- TSH-R; Cancer -- Periodicals; Neoplasms -- Periodicals; Cancer -- Périodiques; Electronic journals; 616.994
• DOI: 10.1016/j.canlet.2014.09.046
• Languages: English
• ISSNs: 0304-3835

Abstract

Abstract: The aim of this study is to determine whether circulating epithelial cells (CEC) detected in patients with differentiated thyroid cancer (DTC) stem from the thyroid gland. CEC have been described to increase in patients with progressive cancer disease and thus have been used as a marker of tumour cell dissemination. CEC were selected from venous blood samples of five DTC patients and analysis of thyroid-specific mRNA (i.e. Tg, TSH-R, TPO, NIS) was performed on a single cell level. 16/48 cells were positive for at least three different thyroid-mRNA transcripts, predominantly found in patients with detectable serum thyroglobulin. In conclusion, evidence was found that in patients with detectable serum thyroglobulin, most of the CECs originate from the thyroid gland. However, further investigations including a larger sample size are needed to validate the clinical impact of this method.