Somatic and neuritic spines on tyrosine hydroxylase–immunopositive cells of rat retina. Issue 7 (13th February 2017)

• Record Type: Journal Article
• Authors: Fasoli, Anna; Dang, James; Johnson, Jeffrey S.; Gouw, Aaron H.; Fogli Iseppe, Alex; Ishida, Andrew T.
• Is Part Of: Journal of comparative neurology. Volume 525:Issue 7(2017)
• Page Start: 1707
• Page End: 1730
• Publication Date: 2017-02-13
• Subjects: retina -- interplexiform cells -- dopamine -- dendrites -- axons -- spines -- RRID: RGD_60991 (organism_LErat) -- RRID: RGD_1566443 (organism_Lrat) -- RRID: AB_2201528 (antibody_TH MS) -- RRID: AB_90755 (antibody_TH_SH) -- RRID: AB_11001825 (antibody_PSD‐93) -- RRID: AB_2307331 (antibody_PSD‐95) -- RRID: AB_399431 (antibody_RIBEYE) -- RRID: AB_2213602 (antibody_GluR1) -- RRID: AB_90711 (antibody_GluR4) -- RRID: AB_2314955 (antibody_NMDAR1) -- RRID: AB_310272 (antibody_GABAA_α1) -- RRID: AB_2340863 (antibody_DKαMSaf647) -- RRID: AB_2315778 (antibody_DKαSHcy3) -- RRID: AB_2338694 (antibody_GTαIgG1MSCy3) -- RRID: AB_2338917 (antibody_GTαIgG2aMSaf647) -- RRID: AB_2576217 (antibody_GTαRBaf488) -- RRID: AB_2338854 (antibody_GTαIgG1MSaf488) -- RRID: SCR_002285 (Fiji) -- RRID: SCR_014237 (Huygens) -- RRID: SCR_007370 (Imaris) -- RRID: SCR_001622 (MatLab) -- RRID: SCR_001905 (R Project for Statistical Computing) -- RRID: NLX_143660 (database_JCNantibody); Comparative neurobiology -- Periodicals; Neurology -- Periodicals; 616
• DOI: 10.1002/cne.24166
• Languages: English
• ISSNs: 0021-9967

Abstract

Abstract: Dopamine‐ and tyrosine hydroxylase–immunopositive cells (TH cells) modulate visually driven signals as they flow through retinal photoreceptor, bipolar, and ganglion cells. Previous studies suggested that TH cells release dopamine from varicose axons arborizing in the inner and outer plexiform layers after glutamatergic synapses depolarize TH cell dendrites in the inner plexiform layer and these depolarizations propagate to the varicosities. Although it has been proposed that these excitatory synapses are formed onto appendages resembling dendritic spines, spines have not been found on TH cells of most species examined to date or on TH cell somata that release dopamine when exposed to glutamate receptor agonists. By use of protocols that preserve proximal retinal neuron morphology, we have examined the shape, distribution, and synapse‐related immunoreactivity of adult rat TH cells. We report here that TH cell somata, tapering and varicose inner plexiform layer neurites, and varicose outer plexiform layer neurites all bear spines, that some of these spines are immunopositive for glutamate receptor and postsynaptic density proteins (viz., GluR1, GluR4, NR1, PSD‐95, and PSD‐93), that TH cell somata and tapering neurites are also immunopositive for a γ‐aminobutyric acid (GABA) receptor subunit (GABAA Rα1 ), and that a synaptic ribbon‐specific protein (RIBEYE) is found adjacent to some colocalizations of GluR1 and TH in the inner plexiform layer. These results identify previously undescribed sites at which glutamatergic and GABAergic inputs may stimulate and inhibit dopamine release, especially at somata and along varicose neurites that emerge from these somata and arborize in various levels of the retina. J. Comp. Neurol. 525:1707–1730, 2017. © 2016 Wiley Periodicals, Inc. Abstract : Using a modified aldehyde fixative, the authors find spines on somata and neurites of tyrosine hydroxylase‐immunopositive interneurons in adult rat retina; colocalizations of glutamate receptor isoform and postsynaptic density proteins at some of these spines, neurites, and somata; and a synaptic ribbon‐specific protein adjacent to some of these colocalizations.