A Photoregulated DNA‐Based Rotary System and Direct Observation of Its Rotational Movement. Issue 16 (15th February 2017)

• Record Type: Journal Article
• Authors: Yang, Yangyang; Tashiro, Ryu; Suzuki, Yuki; Emura, Tomoko; Hidaka, Kumi; Sugiyama, Hiroshi; Endo, Masayuki
• Is Part Of: Chemistry. Volume 23:Issue 16(2017)
• Page Start: 3979
• Page End: 3985
• Publication Date: 2017-02-15
• Subjects: DNA -- high-speed AFM -- molecular wires -- photochemistry -- nanotechnology; Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201605616
• Languages: English
• ISSNs: 0947-6539

Abstract

Abstract: Various DNA‐based nanodevices have been developed on the nanometer scale using light as regulation input. However, the programmed controllability is still a major challenge for these artificial nanodevices. Herein, we demonstrate a rotary DNA nanostructure in which the rotations are controlled by light. A bar‐shaped DNA rotor, fabricated as a stiff double‐crossover molecule, was placed on the top of a rectangular DNA tile. The photoresponsive oligonucleotides modified with azobenzenes were employed as switching motifs to release/trap the rotor at specific angular position on DNA tile by switching photoirradiations between ultraviolet and visible light. As a result, two reconfigurable states (perpendicular and parallel) of rotor were obtained, in which the angular changes were characterized by AFM and fluorescence quenching assays. Moreover, the reversible rotary motions during the photoirradiation were directly visualized on the DNA tile surface in a nanometer‐scale precision using a second‐scale scanning of the high‐speed AFM. Abstract : Spinning around : A DNA‐based rotary mechanical system was constructed and the rotations were controlled by light input. The reversible rotary movements between two distinguished states during the photoirradiation were directly visualized on the DNA origami surface in a nanometer‐scale precision using second‐scale scanning in high speed AFM.