Coupling Activity‐Based Detection, Target Amplification, Colorimetric and Fluorometric Signal Amplification, for Quantitative Chemosensing of Fluoride Generated from Nerve Agents. Issue 16 (15th February 2017)

• Record Type: Journal Article
• Authors: Sun, Xiaolong; Reuther, James F.; Phillips, Scott T.; Anslyn, Eric V.
• Is Part Of: Chemistry. Volume 23:Issue 16(2017)
• Page Start: 3903
• Page End: 3909
• Publication Date: 2017-02-15
• Subjects: colorimetry -- fluorescence -- fluorides -- nerve agents -- sensors; Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201604474
• Languages: English
• ISSNs: 0947-6539

Abstract

Abstract: The G‐class nerve agents, which include sarin, soman, and cyclosarin, react readily with nucleophilic reagents to produce fluoride. Thus, a chemosensing protocol has been designed for these agents that pairs the nucleophilic reactivity of oximates for generating fluoride with an autoinductive target amplification reaction to amplify the quantity of fluoride for facile colorimetric and fluorescent optical quantification. The chemosensing protocol was demonstrated by using the nerve agent surrogate diisopropyl fluorophosphate (DFP) and benzaldoxime as the nucleophile. Autoinductive fluoride amplification responds to fluoride released from DFP by amplifying the fluoride concentration and a yellow reporter molecule. The reporter is a conjugated oligomer with a nominal repeating unit that originates from 4‐aminobenzaldehyde. Exposure of the amplified fluoride to a fluoride‐specific ratiometric fluorescent reporter provides a fluorescent readout, in which three fluorophores are generated per fluoride. Both colorimetric and fluorescent readouts enable quantitative assays with low micromolar limits of detection for fluoride resulting from DFP. More importantly, this work demonstrates the successful merging of multiple complex reactions for achieving selective, sensitive, and quantitative chemosensing. Abstract : Calm your nerve (agents) : A chemosensing protocol for G series nerve agents that pairs the nucleophilic reactivity of oximates for generating fluoride with an autoinductive target amplification reaction to amplify the quantity of fluoride for facile colorimetric and fluorescent optical quantification (see scheme).