Structural insights into the EthR–DNA interaction using native mass spectrometry. Issue 25 (13th March 2017)

• Record Type: Journal Article
• Authors: Shiu-Hin Chan, Daniel; Seetoh, Wei-Guang; McConnell, Brendan N.; Matak-Vinković, Dijana; Thomas, Sherine E.; Mendes, Vitor; Blaszczyk, Michal; Coyne, Anthony G.; Blundell, Tom L.; Abell, Chris
• Is Part Of: Chemical communications. Volume 53:Issue 25(2017)
• Page Start: 3527
• Page End: 3530
• Publication Date: 2017-03-13
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c7cc00804j
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : The interaction between Mycobacterium tuberculosis EthR and its operator DNA has been studied by native mass spectrometry, revealing an interesting stoichiometry. Abstract : EthR is a transcriptional repressor that increases Mycobacterium tuberculosis resistance to ethionamide. In this study, the EthR–DNA interaction has been investigated by native electrospray-ionization mass spectrometry for the first time. The results show that up to six subunits of EthR are able to bind to its operator.