A ratiometric fluorescence probe for imaging sulfur dioxide derivatives in the mitochondria of living cells. Issue 13 (18th January 2017)

• Record Type: Journal Article
• Authors: Wang, Yongfei; Meng, Qingtao; Zhang, Run; Jia, Hongmin; Zhang, Xuehu; Zhang, Zhiqiang
• Is Part Of: Organic & biomolecular chemistry. Volume 15:Issue 13(2017)
• Page Start: 2734
• Page End: 2739
• Publication Date: 2017-01-18
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c6ob02779b
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : The fluorescence probe, NBD-Id, was designed and synthesized for the detection of SO2 derivatives in 100% aqueous solution and living cells. Abstract : Although sulfur dioxide (SO2 ) plays an essential role in several physiological processes, monitoring of intracellular SO2 at subcellular levels remains challenging due to the lack of rapid and sensitive methods for its quantification in a 100% aqueous solution. Herein, a new hemicyanine dyes-based fluorescence probe, NBD-Id, was designed and synthesized for the detection of SO2 derivatives in pure aqueous solution and living cells. By virtue of a specific 1, 4-addition reaction of SO3 2− HSO3 − and the polymethine chain of hemicyanine, significant changes in the absorption and fluorescence emission spectra were observed in less than 20 seconds. The ratiometric fluorescence ( F 467 / F 593 ) detection of SO2 derivatives was then obtained with high sensitivity (detection limit 3.6 nM). It was noted thatNBD-Id has a specific response towards SO2 derivatives without interference from other anions and biomolecules. Intracellular fluorescence imaging indicated thatNBD-Id is cell membrane permeable and mainly distributed within the mitochondria. Therefore, ratiometric fluorescence imaging of SO2 derivatives in the mitochondria of MCF-7 cells was successfully demonstrated.