"Leaching or not leaching": an alternative approach to antimicrobial materials via copolymers containing crown ethers as active groups. (1st March 2017)

• Record Type: Journal Article
• Authors: De Rosa, M.; Vigliotta, G.; Soriente, A.; Capaccio, V.; Gorrasi, G.; Adami, R.; Reverchon, E.; Mella, M.; Izzo, L.
• Is Part Of: Biomaterials science. Volume 5:Number 4(2017:Apr.)
• Page Start: 741
• Page End: 751
• Publication Date: 2017-03-01
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1039/c6bm00950f
• Languages: English
• ISSNs: 2047-4830

Abstract

Abstract : New copolymers containing MMA and 18C6 crown-ether pendants, with or without a PEG arm, were synthesized to check if sequestering structural alkali-earth ions from the bacterial outer membrane (OM) may lead to bacterial death. Abstract : In this work, new copolymers containing either MMA and 18C6 crown-ether pendants, or PEG, MMA and 18C6 crown-ether pendants were synthesized to test the idea that sequestering structural alkali-earth ions from the bacterial outer membrane (OM) may lead to bacterial death. The copolymers were obtained either via uncontrolled radical polymerization or ATRP; the latter approached allowed us to produce not only linear copolymers but also branched Y-like structures. After checking for the capability of complexing magnesium and calcium ions, the antimicrobial activity of all copolymers was tested placing their casted plaques in contact with pure water E. coli suspensions. All plaques adsorbed alkali-earth ions and killed bacteria, albeit with different antimicrobial efficiencies. Differences in the latter characteristic were attributed to different plaque roughness. The role of the 18C6 crown-ether pendants was elucidated by pre-saturating plaques with Mg/Ca ions, the marked reduction in antimicrobial efficiency indicating that losing the latter from OM due to surface complexation does play an important role in killing bacteria at short (<5 h) contact times. At longer times, the mode of action is instead related to the poly-cationic nature acquired by the plaques due to ion sequestering.