The complete mitochondrial genome of the invasive Ponto-Caspian goby Ponticola kessleri obtained from high-throughput sequencing using the Ion Torrent Personal Genome Machine. (3rd May 2016)

• Record Type: Journal Article
• Authors: Kalchhauser, Irene; Kutschera, Verena E.; Burkhardt-Holm, Patricia
• Is Part Of: Mitochondrial DNA. Volume 27:Number 3(2016)
• Page Start: 1887
• Page End: 1889
• Publication Date: 2016-05-03
• Subjects: Bighead goby -- high throughput sequencing -- invasive -- mitochondrial genome -- Ponticola kessleri -- Rhine
• DOI: 10.3109/19401736.2014.971272
• Languages: English
• ISSNs: 2380-2359

Abstract

Abstract: We report the first complete mitochondrial genome (mitogenome) of an invasive Ponto-Caspian goby, Ponticola kessleri (bighead goby, Günther 1891). Ion Torrent PGM sequencing of total DNA from two individuals yielded a contig of 16, 971 bp, with overlapping ends located in the repetitive control region, which was validated using Sanger sequencing. The final mitogenome of Ponticola kessleri has a size of 16, 890 bp and contains the expected gene configuration of 13 protein-coding genes, 2 rRNA genes and 22 tRNA genes. In a comparison with complete mitogenomes from other goby species, we identified a translocation of tRNA-Glu in the mitogenome of P. kessleri. Rearrangements are unique and rare events, and can thus provide phylogenetic information.