Evaluation of capillary electrophoresis for the analysis of rooibos and honeybush tea phenolics. Issue 6 (27th December 2016)

• Record Type: Journal Article
• Authors: Arries, William J.; Tredoux, Andreas G. J.; de Beer, Dalene; Joubert, Elizabeth; de Villiers, André
• Is Part Of: Electrophoresis. Volume 38:Issue 6(2017)
• Page Start: 897
• Page End: 905
• Publication Date: 2016-12-27
• Subjects: Capillary zone electrophoresis -- Herbal tea -- Honeybush -- Phenolics -- Rooibos; Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.201600349
• Languages: English
• ISSNs: 0173-0835

Abstract

Abstract : Rooibos and honeybush are popular herbal teas produced from the shrubs of Aspalathus linearis and Cyclopia spp., respectively, which are indigenous to South Africa. Both herbal teas are rich in polyphenols and their consumption is associated with several health benefits, partly ascribed to their phenolic constituents. Quantification of phenolics in extracts and teas for quality control and research purposes is generally performed using HPLC, although dedicated and often species‐specific methods are required. CE offers an attractive alternative to HPLC for the analysis of phenolics, with potential benefits in terms of efficiency, speed and operating costs. In this contribution, we report quantitative CZE methods for the analysis of the principal honeybush and rooibos phenolics. Optimal separation for honeybush and rooibos phenolics was achieved in 21 and 32 min, respectively, with good linearity and repeatability. Quantitative data for extracts of "unfermented" and "fermented" rooibos and two honeybush species were statistically comparable with those obtained by HPLC for the majority of compounds. The developed methods demonstrated their utility for the comparison of phenolic contents between different species and as a function of manufacturing processes, thus offering cost effective, although less sensitive and robust, alternatives to HPLC analysis.