Isoafricanol synthase from Streptomyces malaysiensis. Issue 11 (1st March 2017)

• Record Type: Journal Article
• Authors: Rabe, Patrick; Samborskyy, Markiyan; Leadlay, Peter F.; Dickschat, Jeroen S.
• Is Part Of: Organic & biomolecular chemistry. Volume 15:Issue 11(2017)
• Page Start: 2353
• Page End: 2358
• Publication Date: 2017-03-01
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c7ob00234c
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : A terpene cyclases from Streptomyces malaysiensis was characterised as (+)-isoafricanol synthase and its mechanism was investigated using isotopically labelled substrates. Abstract : Genome sequencing of Streptomyces malaysiensis DSM 4137 revealed the presence of four terpene cyclase genes, one of which was characterised as (+)-isoafricanol synthase. Its cyclisation mechanism was extensively studied using isotopically labelled precursors. Several enzymes with high homology that likely also function as (+)-isoafricanol synthases are encoded in a number of other genome sequenced streptomycetes.