Turn-on fluorescent probes that can light up endogenous RNA in nucleoli and cytoplasm of living cells under a two-photon microscope. Issue 27 (16th March 2017)

• Record Type: Journal Article
• Authors: Feng, Ruiqing; Li, Longlong; Li, Bing; Li, Jinhui; Peng, Dan; Yu, Yitao; Mu, Qiuhong; Zhao, Ning; Yu, Xiaoqiang; Wang, Zhenhua
• Is Part Of: RSC advances. Volume 7:Issue 27(2017)
• Page Start: 16730
• Page End: 16736
• Publication Date: 2017-03-16
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c6ra28284a
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : We report two turn-on RNA probes that can light up endogenous RNA in the nucleoli and cytoplasm of living cells under a two-photon microscope. Abstract : We have synthesized two-photon organic molecule probes (IMT-E andIMT-M ), which selectively stain endogenous RNA in the nucleolus and cytoplasm of living cells in a short incubation time. Using these probes, bright two-photon excitation fluorescence (TPEF) images of living cells have been developed. Titrations and digest tests of ribonuclease indicate the markedly higher affinity of these probes for RNA, especially when usingIMT-E . Upon binding to RNA, the fluorescence intensity ofIMT-E increases by about 15-fold, showing thatIMT-E is a turn-on probe for the detection of RNAs. MTT assays demonstrate that the mitochondria of cells maintain their electron mediating ability after being stained with the probes. These results demonstrate thatIMT-E is an attractive two-photon turn-on fluorescent probe for visualizing RNA in living cells.