Enhancing ex vivo expansion of cord blood‐derived unrestricted somatic stem cells for clinical applications. (29th October 2013)

• Record Type: Journal Article
• Authors: Demerdash, Z.; El Baz, H.; Mahmoud, F.; Mohamed, S.; Maher, K.; Gaafar, T.; Shawky, S.; Hassan, M.; Abdelhady, D.; Taha, T.
• Is Part Of: Cell proliferation. Volume 46:Number 6(2013:Dec.)
• Page Start: 628
• Page End: 636
• Publication Date: 2013-10-29
• Subjects: Cell proliferation -- Periodicals; 571.84
• DOI: 10.1111/cpr.12070
• Languages: English
• ISSNs: 0960-7722

Abstract

Abstract: Objectives: To study effects of serum‐containing medium (SCM) versus serum‐free medium (SFM) and influence of seeding density, on rate of expansion of cord blood (CB) unrestricted somatic stem cells (USSCs), as a prerequisite for evaluating their therapeutic potential in ongoing clinical trials. Material and methods: Isolation, propagation and characterization of USSCs from CB samples were performed and followed by their passage 3 culture in SCM and SFM, at cell densities of 5, 50, 500 and 5000 cells/cm 2 . Results: The cells were CD44 +, CD90 +, CD73 +, CD105 +, CD34 −, CD45 −, and HLA‐DR, with Oct4 & Sox2 gene expression; they were differentiated into osteoblasts and adipocytes. USSCs cultured in SCM had significantly higher population doubling levels ( P < 0.01) than those cultured in SFM. Those cultured in SCM at 5 cells/cm 2 and those cultured in SFM at 50 cells/cm 2 had significantly higher population doubling ( P < 0.01) levels than those cultured at higher cell densities. Conclusions: For scaling up of USSCs from 106 (?) to 1012 (?) in 6 weeks, culturing of CB‐derived cells of early passage (≤P3) in SCM at low cell seeding density (5 cells/cm 2 ) is suggested for increasing cell count with lower passaging frequency, followed by culture of expanded USSCs at 50 cells/cm 2 in SFM, to avoid undesirable effects of bovine serum in clinical applications.