TNF‐α triggers osteogenic differentiation of human dental pulp stem cells via the NF‐κB signalling pathway. (21st October 2013)

• Record Type: Journal Article
• Authors: Feng, Xingmei; Feng, Guijuan; Xing, Jing; Shen, Biyu; Li, Liren; Tan, Wei; Xu, Yue; Liu, Suzhe; Liu, Hong; Jiang, Jinxia; Wu, Hao; Tao, Tao; Gu, Zhifeng
• Is Part Of: Cell biology international. Volume 37:Number 12(2013)
• Page Start: 1267
• Page End: 1275
• Publication Date: 2013-10-21
• Subjects: dental pulp stem cells (DPSCs) -- nuclear factor kappaB (NF‐κB) -- osteogenic differentiation -- tumour necrosis factor α (TNF‐α); Cytology -- Periodicals; Cells -- Periodicals; 571.605
• DOI: 10.1002/cbin.10141
• Languages: English
• ISSNs: 1065-6995

Abstract

Abstract: Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cells (MSCs) characterised by self‐renewal and multi‐lineage differentiation, including chondrocytes, adipocytes, neural cells and osteoblasts, which make it an attractive choice for tissue engineering purposes. Tumour necrosis factor α (TNF‐α) had the positive effect on the mineralisation of bone marrow MSCs and stromal cells derived from human adipose tissue. However, the effect of TNF‐α on DPSCs is unclear. We found that TNF‐α activated the NF‐κB pathway during the osteogenic differentiation of DPSCs. TNF‐α also increased mineralisation and the expression of bone morphogenetic protein 2 (BMP2), alkaline phosphatase (ALP), runt‐related transcription factor 2 (RUNX2) and collagen type I (COL I) during this process. PDTC, an NF‐κB inhibitor, blocked the osteogenic differentiation induced by TNF‐α. No effect of TNF‐α on proliferation of DPSCs or cell cycle was detected. In summary, TNF‐α promotes mineralisation and mineralisation‐related gene expression through the NF‐κB signalling pathway in DPSCs, which may provide a foundation for autologous transplantation of DPSCs.