Isolation and Quantification of Phenolic Compounds in Acer tegmentosum by High Performance Liquid Chromatography. (22nd February 2017)

• Record Type: Journal Article
• Authors: Lee, JungIn; Hwang, In Hyun; Jang, Tae Su; Na, MinKyun
• Is Part Of: Bulletin of the Korean Chemical Society. Volume 38:Number 3(2017)
• Page Start: 392
• Page End: 396
• Publication Date: 2017-02-22
• Subjects: Acer tegmentosum -- Quantification method -- High performance liquid chromatography; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/bkcs.11099
• Languages: English
• ISSNs: 0253-2964

Abstract

Abstract : Determination of bioactive chemicals in Acer tegmentosum is essential for its industrial application. Twelve phenolic compounds were isolated from A. tegmentosum . Specific seven compounds in the plant were simultaneously analyzed by high performance liquid chromatography‐UV. The content of bioactive chemicals in the branch extract was determined to be 3.38 mg/g of (−)‐epigallocatechin, 66.95 mg/g of salidroside, 39.61 mg/g of tyrosol, 17.04 mg/g of catechin, 7.39 mg/g of 1, 2, 4‐tri‐ O ‐galloyl‐β‐d ‐glucose, 8.88 mg/g of 6‐ O ‐galloylsalidroside, and 4.61 mg/g of scopoletin. The method was validated with linearity ( R 2 > 0.9947), as well as intra‐ and inter‐day precision (0.07–2.77%) and accuracy (>97.0%). This analytical method is expected to be applied for standardization of dietary food containing A. tegmentosum . Abstract :