Identification of Optimal Reference Genes for Normalization of RT-qPCR Data in Cancerous and Non-Cancerous Tissues of Human Uterine Cervix. (16th March 2017)

• Record Type: Journal Article
• Authors: Tan, Shing Cheng; Ismail, Mohd Pazudin; Duski, Daniel Roza; Othman, Nor Hayati; Bhavaraju, Venkata Murali Krishna; Ankathil, Ravindran
• Is Part Of: Cancer investigation. Volume 35:Number 3(2017)
• Page Start: 163
• Page End: 173
• Publication Date: 2017-03-16
• Subjects: Endogenous control genes -- gene expression -- normalization -- quantitative real-time PCR -- reference genes -- uterine cervical neoplasms; Cancer -- Periodicals; Oncology -- Periodicals; Medical Oncology -- Periodicals; Neoplasms -- Periodicals; 616.994
• DOI: 10.1080/07357907.2017.1278767
• Languages: English
• ISSNs: 0735-7907

Abstract

ABSTRACT: This study aimed to identify the most stably expressed reference genes from a panel of 32 candidate genes for normalization of reverse transcription-quantitative real-time polymerase chain reaction data in cancerous and non-cancerous tissues of human uterine cervix. Overall, PUM1, YWHAZ, and RPLP0 were identified as the most stably expressed genes in paired cancerous and non-cancerous tissues. The results were further stratified by the state of malignancy of the tissues, histopathological type of the cancer, and the human papillomavirus-type.