Rabbit monoclonal anti-SATB2: identification of malignancies from lower GI tract. (2nd January 2017)

• Record Type: Journal Article
• Authors: Cummings, Kelsea; Lorge, Rebecca; Merritt, Brett; Gordon, Jeffrey; Minasyan, Ashot; Yang, George; Su, Qin
• Is Part Of: Journal of histotechnology. Volume 40:Number 1(2017)
• Page Start: 9
• Page End: 12
• Publication Date: 2017-01-02
• Subjects: Antibodies -- Colorectal carcinoma -- Diagnosis -- Immunohistochemistry -- Lower gastrointestinal -- Pathology -- SATB2; Histology -- Periodicals; Cytology -- Periodicals; 611.018
• DOI: 10.1080/01478885.2016.1224289
• Languages: English
• ISSNs: 0147-8885

Abstract

Abstract : The differential diagnosis of metastases with no known history or a poorly differentiated subtype may present a diagnostic challenge – especially for small biopsy specimens. This study evaluated rabbit monoclonal anti-SATB2 by immunohistochemistry for neoplastic expression, sensitivity, and specificity. Ninety-five percent of primary colorectal adenocarcinoma cases displayed strong and diffuse nuclear staining with rabbit monoclonal anti-SATB2. Anti-SATB2 showed diffuse, weak, and nuclear expression in a case of medullary carcinoma of the colon, while other common colorectal markers showed no immunoreactivity. Anti-SATB2 also showed strong and diffuse nuclear staining in colorectal adenocarcinoma metastases to uterus, liver, lymph nodes, and lung (11/11, 100%). SATB2 rabbit monoclonal antibody is a sensitive and specific marker for malignancies of lower GI origin and is useful for differential diagnosis of metastatic adenocarcinomas of unknown primary.