Novel artificial metalloenzymes by in vivo incorporation of metal-binding unnatural amino acids. Issue 1 (15th October 2014)

• Record Type: Journal Article
• Authors: Drienovská, Ivana; Rioz-Martínez, Ana; Draksharapu, Apparao; Roelfes, Gerard
• Is Part Of: Chemical science. Volume 6:Issue 1(2015:Jan.)
• Page Start: 770
• Page End: 776
• Publication Date: 2014-10-15
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c4sc01525h
• Languages: English
• ISSNs: 2041-6520

Abstract

Abstract : Artificial metalloenzymes for asymmetric catalysis were created by in vivo incorporation of unnatural metal binding amino acids into LmrR. Abstract : Artificial metalloenzymes have emerged as an attractive new approach to enantioselective catalysis. Herein, we introduce a novel strategy for preparation of artificial metalloenzymes utilizing amber stop codon suppression methodology for the in vivo incorporation of metal-binding unnatural amino acids. The resulting artificial metalloenzymes were applied in catalytic asymmetric Friedel–Crafts alkylation reactions and up to 83% ee for the product was achieved.