Nicotinamide downregulates gene expression of interleukin‐6, interleukin‐10, monocyte chemoattractant protein‐1, and tumour necrosis factor‐α gene expression in HaCaT keratinocytes after ultraviolet B irradiation. (12th February 2013) - Monfrecola, G.
Summary: Ultraviolet (UV) radiation has profound effects on human skin, causing sunburn, inflammation, cellular‐tissue injury, cell death, and skin cancer. Most of these effects are mediated by a number of cytokines produced by keratinocytes. In this study we investigated whether nicotinamide (NCT), the amide form of vitamin B3, might have a protective function in reducing the expression of interleukin (IL)‐1β, IL‐6, IL‐8, IL‐10, monocyte chemoattractant protein (MCP)‐1 and tumour necrosis factor (TNF)‐α in UV‐irradiated keratinocytes. HaCaT cells were treated with UVB in the presence or absence of NCT, and cytokine mRNA levels were examined by quantitative real‐time PCR. NCT significantly downregulated IL‐6, IL‐10, MCP‐1 and TNF‐α mRNA expression, whereas it did not exert any significant effect on IL‐1β or IL‐8 expression. Because of its ability to decrease these cytokine mediators after UV exposure, NCT is a possible therapy to improve or prevent conditions induced or aggravated by UV light.