Calcification of aortic human valves studied in situ by Raman microimaging: following mineralization from small grains to big deposits. (15th August 2013)

• Record Type: Journal Article
• Authors: Pilarczyk, Marta; Czamara, Krzysztof; Baranska, Malgorzata; Natorska, Joanna; Kapusta, Przemysław; Undas, Anetta; Kaczor, Agnieszka
• Is Part Of: Journal of Raman spectroscopy. Volume 44:Number 9(2013:Sep.)
• Page Start: 1222
• Page End: 1229
• Publication Date: 2013-08-15
• Subjects: 3D imaging -- aortic stenosis -- calcium salts -- confocal Raman microscopy -- depth profiling; Raman spectroscopy -- Periodicals; 535.846
• DOI: 10.1002/jrs.4352
• Languages: English
• ISSNs: 0377-0486

Abstract

Abstract: A Raman microimaging‐based approach has been used in the current study to evaluate formation and progression of calcification in situ in human stenotic aortic valves obtained during surgical valve replacement. The capability of the method to visualize distribution of the calcified deposits resulted in structural characterization of deposits in the various phases of development. A high spatial resolution of the method along with the confocal depth profiling enabled to identify extremely small salt inclusions (of ca . 0.5 µm in diameter), formed probably at the very early stage of calcification. Structurally, these inclusions are built from an octacalcium phosphate‐like compound that during grains' growth transforms into tricalcium phosphate, mixed with the salt containing the acidic phosphate groups (HPO4 2− ) and, finally, into stable B‐type hydroxyapatite that is the only salt present in large‐area calcium salt deposits. Copyright © 2013 John Wiley & Sons, Ltd. Abstract : Raman microimaging has been used to evaluate formation and progression of calcification in situ in human stenotic aortic valves. The capability of the method to visualize distribution of the calcified deposits resulted in structural characterization of mineralization in the various phases of development.