Chemoenzymatic synthesis of para-nitrophenol (pNP)-tagged α2–8-sialosides and high-throughput substrate specificity studies of α2–8-sialidases. Issue 1 (7th December 2016)

• Record Type: Journal Article
• Authors: Tasnima, Nova; Yu, Hai; Li, Yanhong; Santra, Abhishek; Chen, Xi
• Is Part Of: Organic & biomolecular chemistry. Volume 15:Issue 1(2017)
• Page Start: 160
• Page End: 167
• Publication Date: 2016-12-07
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c6ob02240e
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : para -Nitrophenyl α2–8-sialosides containing different sialic acid forms were chemoenzymatically synthesized and used as effective probes for high-throughput substrate specificity studies of the α2–8-sialidase activities of human and bacterial sialidases. Abstract : para -Nitrophenol ( p NP)-tagged α2–8-linked sialosides containing different sialic acid forms were chemoenzymatically synthesized using an efficient one-pot three-enzyme α2–8-sialylation system. The resulting compounds allowed high-throughput substrate specificity studies of the α2–8-sialidase activity of a recombinant human cytosolic sialidase hNEU2 and various bacterial sialidases. The sialoside substrate profiles obtained can be used to guide the selection of suitable sialidases for sialylglycan analysis and for cell and tissue surface glycan modification. They can also be used to guide sialidase inhibitor design.