Syntheses of polypyridyl metal complexes and studies of their interaction with quadruplex DNA. Issue 8 (31st October 2014)

• Record Type: Journal Article
• Authors: Stafford, Verity S.; Suntharalingam, Kogularamanan; Shivalingam, Arun; White, Andrew J. P.; Mann, David J.; Vilar, Ramon
• Is Part Of: Dalton transactions. Volume 44:Issue 8(2015)
• Page Start: 3686
• Page End: 3700
• Publication Date: 2014-10-31
• Subjects: Chemistry, Inorganic -- Periodicals; Chemistry, Physical and theoretical -- Periodicals; Chemistry, Inorganic -- Periodicals; 546.05
• DOI: 10.1039/c4dt02910k
• Languages: English
• ISSNs: 1477-9226

Abstract

Abstract : A series of metal complexes with substituted terpyridines show high affinity towards quadruplex DNA, particularly bimetallic (trpy)Pt/M(cyclen) complexes. Abstract : A series of mono- and bi-metallic metal complexes (with Cu II, Pt II and Zn II ) with substituted polypyridyl ligands have been prepared and their binding affinities towards quadruplex ( c-Myc and human telomeric) and duplex DNA (ds26 and calf thymus) determined using fluorescent indicator displacement (FID) assays and UV/vis spectroscopic titrations. These studies have shown that the number of aromatic rings and number/position of cyclic amine substituents on the ligands, play an important role in defining the DNA binding abilities of the resulting metal complexes. We also show that bi-metallic complexes prepared using a novel terpyridine-cyclen ligand have higher affinity towards G-quadruplex DNA as compared to their mono-metallic counterparts. Cytotoxicity assays were carried out for all the new complexes against an osteosarcoma cancer cell line (U2OS) as well as a normal fibroblast cell line (GM05757). Several of these compounds displayed cytotoxicity similar to that of cisplatin.