Antimicrobial peptide coatings for hydroxyapatite: electrostatic and covalent attachment of antimicrobial peptides to surfaces. Issue 126 (January 2017)

• Record Type: Journal Article
• Authors: Townsend, Leigh; Williams, Richard L.; Anuforom, Olachi; Berwick, Matthew R.; Halstead, Fenella; Hughes, Erik; Stamboulis, Artemis; Oppenheim, Beryl; Gough, Julie; Grover, Liam; Scott, Robert A. H.; Webber, Mark; Peacock, Anna F. A.; Belli, Antonio; Logan, Ann; de Cogan, Felicity
• Is Part Of: Journal of the Royal Society interface. Volume 14:Issue 126(2017)
• Publication Date: 2017-01
• Subjects: surface engineering -- antimicrobial -- device–tissue interface; Physical sciences -- Research -- Periodicals; Life sciences -- Research -- Periodicals; Interdisciplinary research -- Periodicals; 570.5
• DOI: 10.1098/rsif.2016.0657
• Languages: English
• ISSNs: 1742-5689

Abstract

Abstract : The interface between implanted devices and their host tissue is complex and is often optimized for maximal integration and cell adhesion. However, this also gives a surface suitable for bacterial colonization. We have developed a novel method of modifying the surface at the material–tissue interface with an antimicrobial peptide (AMP) coating to allow cell attachment while inhibiting bacterial colonization. The technology reported here is a dual AMP coating. The dual coating consists of AMPs covalently bonded to the hydroxyapatite surface, followed by deposition of electrostatically bound AMPs. The dual approach gives an efficacious coating which is stable for over 12 months and can prevent colonization of the surface by both Gram-positive and Gram-negative bacteria.