Using high‐throughput in situ plate screening to evaluate the effect of dehydration on protein crystals. (1st May 2013)

• Record Type: Journal Article
• Authors: Douangamath, Alice; Aller, Pierre; Lukacik, Petra; Sanchez‐Weatherby, Juan; Moraes, Isabel; Brandao‐Neto, Jose
• Is Part Of: Acta crystallographica. Volume 69:Part 5(2013:May)
• Page Start: 920
• Page End: 923
• Publication Date: 2013-05-01
• Subjects: high throughput -- in situ plate screening -- dehydration; Biomolecules -- Structure -- Periodicals; Physical biochemistry -- Periodicals; X-ray crystallography -- Periodicals; Crystallography -- Periodicals; 572
• DOI: 10.1107/S0907444913002412
• Languages: English
• ISSNs: 0907-4449

Abstract

Abstract : Crystal dehydration is a post‐crystallization technique that can potentially improve the diffraction of macromolecular crystals. There are currently several ways of undertaking this process; however, dehydration experiments are often limited in their throughput and require prior manipulation of the samples. In the present study, a novel method is proposed that uses in situ plate screening to assess the effect of dehydration by combining the throughput of 96‐well crystallization plates with direct X‐ray feedback on crystal diffraction quality.