DNA G‐quadruplexes show strong interaction with DNA methyltransferases in vitro. Issue 17 (11th August 2016)

• Record Type: Journal Article
• Authors: Cree, Simone L.; Fredericks, Rayleen; Miller, Allison; Pearce, F. Grant; Filichev, Vyacheslav; Fee, Conan; Kennedy, Martin A.
• Is Part Of: FEBS letters. Volume 590:Issue 17(2016)
• Page Start: 2870
• Page End: 2883
• Publication Date: 2016-08-11
• Subjects: DNA methylation -- genomic imprinting -- G‐quadruplex -- protein–DNA interaction; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.12331
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : The DNA methyltransferase enzymes (DNMTs) catalyzing cytosine methylation do so at specific locations of the genome, although with some level of redundancy. The de novo methyltransferases DNMT3A and 3B play a vital role in methylating the genome of the developing embryo in regions devoid of methylation marks. The ability of DNMTs to colocalize at sites of DNA damage is suggestive that recognition of mispaired bases and unusual structures is inherent to the function of these proteins. We provide evidence for G‐quadruplex formation within imprinted gene promoters, and report high‐affinity binding of recombinant human DNMTs to such DNA G‐quadruplexes in vitro . These observations suggest a potential interaction of G‐quadruplexes with the DNA methylation machinery, which may be of epigenetic and biological significance. Abstract :