A rapid response "Turn-On" fluorescent probe for nitroreductase detection and its application in hypoxic tumor cell imaging. Issue 2 (25th November 2014)

• Record Type: Journal Article
• Authors: Xu, Jian; Sun, Shaobo; Li, Qian; Yue, Ying; Li, Yingdong; Shao, Shijun
• Is Part Of: Analyst. Volume 140:Issue 2(2015)
• Page Start: 574
• Page End: 581
• Publication Date: 2014-11-25
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c4an01934b
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A novel "Turn-On" fluorescent probe, quaternarized 4-pyridinyl-substituted BODIPY dye by incorporating a 5-nitrofuran moiety, was developed and applied for imaging the hypoxic status of tumor cells by the indirect detection of nitroreductase. Abstract : A novel "Turn-On" fluorescent probe, quaternarized 4-pyridinyl-substituted BODIPY dye by incorporating a 5-nitrofuran moiety, was developed and applied for imaging the hypoxic status of tumor cells by the indirect detection of nitroreductase. The design was based on a nitroreductase-catalyzed reduction of the nitrofuran moiety in the presence of reduced nicotinamide adenine dinucleotide (NADH) as an electron donor and followed by the 1, 6-rearrangement-elimination and the release of free 4-pyridinyl-substituted BODIPY dye2 . This probe displayed desired properties such as high specificity, "Turn-On" fluorescence response with suitable sensitivity, appreciable water solubility and rapid response time (within 5 min). Moreover, as a biocompatible molecule, the probe has been successfully applied for imaging the hypoxic status of tumor cells ( e.g. A549 cells) and especially used for real-time determination of nitroreductase produced by Escherichia coli . Therefore, we hope to apply this novel method in the biomedical research fields for the imaging of disease-relevant hypoxia and detection of pathogenic microorganisms.