Marker-free automated histopathological annotation of lung tumour subtypes by FTIR imaging. Issue 7 (22nd December 2014)

• Record Type: Journal Article
• Authors: Großerueschkamp, Frederik; Kallenbach-Thieltges, Angela; Behrens, Thomas; Brüning, Thomas; Altmayer, Matthias; Stamatis, Georgios; Theegarten, Dirk; Gerwert, Klaus
• Is Part Of: Analyst. Volume 140:Issue 7(2015)
• Page Start: 2114
• Page End: 2120
• Publication Date: 2014-12-22
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c4an01978d
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : Automated detection of lung cancer adenocarcinoma subtypes by FTIR imaging is presented in this study for the first time. Abstract : By integration of FTIR imaging and a novel trained random forest classifier, lung tumour classes and subtypes of adenocarcinoma are identified in fresh-frozen tissue slides automated and marker-free. The tissue slices are collected under standard operation procedures within our consortium and characterized by current gold standards in histopathology. In addition, meta data of the patients are taken. The improved standards on sample collection and characterization results in higher accuracy and reproducibility as compared to former studies and allows here for the first time the identification of adenocarcinoma subtypes by this approach. The differentiation of subtypes is especially important for prognosis and therapeutic decision.