The best of both worlds: active enzymes by grafting-to followed by grafting-from a protein. Issue 25 (22nd December 2014)

• Record Type: Journal Article
• Authors: Falatach, Rebecca; McGlone, Cameron; Al-Abdul-Wahid, M. Sameer; Averick, Saadyah; Page, Richard C.; Berberich, Jason A.; Konkolewicz, Dominik
• Is Part Of: Chemical communications. Volume 51:Issue 25(2015)
• Page Start: 5343
• Page End: 5346
• Publication Date: 2014-12-22
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c4cc09287b
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Hydrophilic polymers were attached to lysozyme by a combination of grafting-to and grafting-from approaches using RAFT polymerization. Abstract : Hydrophilic polymers were attached to lysozyme by a combination of grafting-to and grafting-from approaches using RAFT polymerization. A hydrophilic oligomer was synthesized, and attached to the protein. The protein–oligomer hybrid contained the RAFT end group, enabling chain extension in solution. Lysozyme maintained activity throughout this process.