Effective CpG DNA delivery using amphiphilic cycloamylose nanogels. (13th October 2014)

• Record Type: Journal Article
• Authors: Tahara, Y.; Yasuoka, J.; Sawada, S.; Sasaki, Y.; Akiyoshi, K.
• Is Part Of: Biomaterials science. Volume 3:Number 2(2015:Feb.)
• Page Start: 256
• Page End: 264
• Publication Date: 2014-10-13
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1039/c4bm00293h
• Languages: English
• ISSNs: 2047-4830

Abstract

Abstract : Native CpG oligodeoxynucleotides induce inflammatory immune responses through cytokine production by their effective cell uptake with amphiphilic cationic cycloamylose nanogels. Abstract : Unmethylated CpG oligodeoxynucleotides induce inflammatory immune responses through cytokine production and have attracted increasing attention as an immunostimulator. However, there remains a challenging issue of the use of 'native CpG DNA'. In the present study, we prepared cationic nanometer-sized gels (nanogels) consisting of cycloamylose modified with cholesterol and diethylaminoethane to form hydrophobic cross-linking points and to add positively charged groups, respectively. The cationic nanogels and native CpG DNA formed nanometer-sized complexes. Complexes of native CpG DNA with cationic nanogels delivered native CpG DNA to macrophage-like cells and induced cytokine production. In addition, complexes of negative control oligonucleotides with cationic nanogels did not induce cytokine production, and the induction of cytokines using complexes of phosphorothioate-modified CpG with cationic nanogels was lower than that of native CpG DNA. These results suggest that the complex of native CpG DNA with cationic nanogels is a promising strategy for nucleic acid adjuvants.